LutefiskXP LutefiskXP is an ANSI C compliant software used to perform the new peptide sequencing from tandem mass spectra.
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peptide peptide sequencing tandem mass spectra sequencing tandem

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LutefiskXP is an ANSI C compliant software used to perform the new peptide sequencing from tandem mass spectra. LutefiskXP is an ANSI C compliant software used to perform the new peptide sequencing from tandem mass spectra. Lutefisk is software for the de novo interpretation of peptide CID spectra. High quality tandem mass spectra of peptides are often obtained for which no exact database match can be made.Consequently, we are faced with the question of whether the protein under investigation is novel, or if the non-matching spectra are due to less exciting prospects such as inter-species variation, database sequence errors, or unexpected proteolytic cleavages. To begin addressing this problem we perform a de novointerpretation of the CID spectra using the computer program Lutefisk; however, any such interpretations nearly always yield multiple sequence candidates, where it is often difficult or impossible to distinguish the correct sequence from the incorrect ones.The variations between candidate sequences are often minor and typically involve dipeptide inversions, swapping of dipeptides of the same mass, replacements of dipeptides with single amino acids of the same mass, and replacements of amino acids by dipeptides of the same mass. We use the multiple sequence candidates produced by Lutefisk as query sequences in a second program, CIDentify. CIDentify is a version of Bill Pearson's FASTA algorithm modified by Alex Taylor to accommodate MS nuances such as multiple query sequences, ambiguous dipeptides and isobaric mass equivalencies.Compiling on UNIXAfter untarring the archive, copy the makefile for your system to "Makefile". Use the "make lutefisk" command.USAGE: lutefisk -o = output file pathname -q = quiet mode ON (default OFF) -m = precursor ion mass -d = details file pathname -p = params file pathname -r = residues file pathname -s = pathnane of file with database sequences to score -v = verbose mode ON (default OFF) -h = print this help textWhat's New in This Release:· Updated makefile

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